Current advances in methodologies to study carbohydrate metabolism and glycobiology in health and disease. Changes in carbon flux and glycoprotein formation play a major role in the development of diseases such as cancer. Modern technologies such as Seahorse Extracellular Flux (XF) analysis and Fourier Transform Infra-Red (FTIR) spectroscopy are being applied to better understand human pathogenesis in cancer. Seahorse XF can simultaneously measure the mitochondrial oxygen consumption rate and the extracellular acidification rate corresponding to anaerobic glycolysis, while FTIR spectroscopy measures carbohydrate moieties on glycoproteins. Write an essay on the role of carbon metabolism and glycan formation in healthy and cancer cells. Explain in your essay how technologies such as Seahorse Extracellular Flux (XF) analysis and Fourier Transform Infra-Red (FTIR) spectroscopy have provided novel insights into human pathologies in cancer. Describe at least one example for each technology. Your answer should not describe the principles of the technologies but instead, focus on their applications.
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